PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT2G46400.1
Common NameATWRKY46, F11C10.9, WRKY46
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family WRKY
Protein Properties Length: 295aa    MW: 33634.7 Da    PI: 5.9371
Description WRKY DNA-binding protein 46
Gene Model
Gene Model ID Type Source Coding Sequence
AT2G46400.1genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1WRKY89.33.3e-28104164259
                  --SS-EEEEEEE--TT-SS-EEEEEE-ST...T---EEEEEE-SSSTTEEEEEEES--SS- CS
         WRKY   2 dDgynWrKYGqKevkgsefprsYYrCtsa...gCpvkkkversaedpkvveitYegeHnhe 59 
                  dDg++WrKYGqKe++gs++pr+YYrCt++   +C + k+v++s++dp+++e++Y g+H+++
  AT2G46400.1 104 DDGHCWRKYGQKEIHGSKNPRAYYRCTHRftqDCLAVKQVQKSDTDPSLFEVKYLGNHTCN 164
                  8***************************9999***************************97 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
Gene3DG3DSA:2.20.25.801.1E-2690165IPR003657WRKY domain
PROSITE profilePS5081121.02398161IPR003657WRKY domain
SuperFamilySSF1182904.97E-2498164IPR003657WRKY domain
SMARTSM007746.1E-36103165IPR003657WRKY domain
PfamPF031066.5E-23104163IPR003657WRKY domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0010200Biological Processresponse to chitin
GO:0048527Biological Processlateral root development
GO:0005730Cellular Componentnucleolus
GO:0000987Molecular Functioncore promoter proximal region sequence-specific DNA binding
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009005anatomyroot
PO:0009006anatomyshoot system
PO:0009009anatomyplant embryo
PO:0009010anatomyseed
PO:0009025anatomyvascular leaf
PO:0009029anatomystamen
PO:0009030anatomycarpel
PO:0009031anatomysepal
PO:0009032anatomypetal
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0009052anatomyflower pedicel
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0025281anatomypollen
PO:0001054developmental stagevascular leaf senescent stage
PO:0001078developmental stageplant embryo cotyledonary stage
PO:0001081developmental stagemature plant embryo stage
PO:0001185developmental stageplant embryo globular stage
PO:0004507developmental stageplant embryo bilateral stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
PO:0007616developmental stageflowering stage
Sequence ? help Back to Top
Protein Sequence    Length: 295 aa     Download sequence    Send to blast
MMMEEKLVIN ELELGKELAN RLMNNLKHTS SVDSNKTLIS DILRIYQNAI FMLSFNQDKN  60
ILKRSLEIDG KDSKNVFKKR KVSEKNTEKV KVFVATEQEN GSIDDGHCWR KYGQKEIHGS  120
KNPRAYYRCT HRFTQDCLAV KQVQKSDTDP SLFEVKYLGN HTCNNITSPK TTTNFSVSLT  180
NTNIFEGNRV HVTEQSEDMK PTKSEEVMIS LEDLENKKNI FRTFSFSNHE IENGVWKSNL  240
FLGNFVEDLS PATSGSAITS EVLSAPAAVE NSETADSYFS SLDNIIDFGQ DWLWS
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.265100.0root| seed
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible263783_at0.0
Expression AtlasAT2G46400-
AtGenExpressAT2G46400-
ATTED-IIAT2G46400-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Expressed in guard cells, hypocotyls, and in the vascular tissues of cotyledon and root. {ECO:0000269|PubMed:24773321}.
Functional Description ? help Back to Top
Source Description
TAIRmember of WRKY Transcription Factor; Group III
UniProtTranscription factor involved in the regulation of osmotic stress responses and stomatal movement (PubMed:24773321). Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). {ECO:0000250, ECO:0000269|PubMed:24773321}.
Function -- GeneRIF ? help Back to Top
  1. WRKY28 and WRKY46 are transcriptional activators of ICS1 and PBS3, respectively, and regulate salicylic acid synthesis upon pathogen attack.
    [PMID: 21595875]
  2. WRKY46, WRKY70, and WRKY53 positively regulate basal resistance to Pseudomonas syringae; and that they play overlapping and synergetic roles in plant basal defense.
    [PMID: 22325892]
  3. WRKY46 functions in Al sensitivity through regulating the expression of ALMT1. Mutation of WRKY46 leads to increased Al resistance.
    [PMID: 24118304]
  4. WRKY46 plays dual roles in regulating plant responses to drought and salt stress and light-dependent stomatal opening in guard cells.
    [PMID: 24773321]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00112ampDAP27203113Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT2G46400.1
Regulation -- Description ? help Back to Top
Source Description
UniProtINDUCTION: Up-regulated by drought, salt and hydrogen peroxide treatments. {ECO:0000269|PubMed:24773321}.
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Phenotype -- Disruption Phenotype ? help Back to Top
Source Description
UniProtDISRUPTION PHENOTYPE: Decreased tolerance to dehydration and salt stress. {ECO:0000269|PubMed:24773321}.
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT2G46400
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAY0462750.0AY046275.1 Arabidopsis thaliana WRKY transcription factor 46 (WRKY46) mRNA, complete cds.
GenBankAY0808120.0AY080812.1 Arabidopsis thaliana putative WRKY-type DNA binding protein (At2g46400) mRNA, complete cds.
GenBankAY1172100.0AY117210.1 Arabidopsis thaliana putative WRKY-type DNA binding protein (At2g46400) mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_182163.10.0putative WRKY transcription factor 46
SwissprotQ9SKD90.0WRK46_ARATH; Probable WRKY transcription factor 46
TrEMBLD7LDZ60.0D7LDZ6_ARALL; Putative uncharacterized protein
STRINGAT2G46400.10.0(Arabidopsis thaliana)
Orthologous Group ? help Back to Top
LineageOrthologous Group IDTaxa NumberGene Number
MalvidsOGEM48882352
Representative plantOGRP1417875
Publications ? help Back to Top
  1. Eulgem T,Rushton PJ,Robatzek S,Somssich IE
    The WRKY superfamily of plant transcription factors.
    Trends Plant Sci., 2000. 5(5): p. 199-206
    [PMID:10785665]
  2. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  3. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  4. Zhu J, et al.
    An Arabidopsis homeodomain transcription factor gene, HOS9, mediates cold tolerance through a CBF-independent pathway.
    Proc. Natl. Acad. Sci. U.S.A., 2004. 101(26): p. 9873-8
    [PMID:15205481]
  5. Guan Y,Nothnagel EA
    Binding of arabinogalactan proteins by Yariv phenylglycoside triggers wound-like responses in Arabidopsis cell cultures.
    Plant Physiol., 2004. 135(3): p. 1346-66
    [PMID:15235117]
  6. Hass C, et al.
    The response regulator 2 mediates ethylene signalling and hormone signal integration in Arabidopsis.
    EMBO J., 2004. 23(16): p. 3290-302
    [PMID:15282545]
  7. Chang S,Pikaard CS
    Transcript profiling in Arabidopsis reveals complex responses to global inhibition of DNA methylation and histone deacetylation.
    J. Biol. Chem., 2005. 280(1): p. 796-804
    [PMID:15516340]
  8. Koroleva OA,Tomlinson ML,Leader D,Shaw P,Doonan JH
    High-throughput protein localization in Arabidopsis using Agrobacterium-mediated transient expression of GFP-ORF fusions.
    Plant J., 2005. 41(1): p. 162-74
    [PMID:15610358]
  9. Lee BH,Henderson DA,Zhu JK
    The Arabidopsis cold-responsive transcriptome and its regulation by ICE1.
    Plant Cell, 2005. 17(11): p. 3155-75
    [PMID:16214899]
  10. Suzuki N, et al.
    Enhanced tolerance to environmental stress in transgenic plants expressing the transcriptional coactivator multiprotein bridging factor 1c.
    Plant Physiol., 2005. 139(3): p. 1313-22
    [PMID:16244138]
  11. Taki N, et al.
    12-oxo-phytodienoic acid triggers expression of a distinct set of genes and plays a role in wound-induced gene expression in Arabidopsis.
    Plant Physiol., 2005. 139(3): p. 1268-83
    [PMID:16258017]
  12. Stein M, et al.
    Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration.
    Plant Cell, 2006. 18(3): p. 731-46
    [PMID:16473969]
  13. Truman W,de Zabala MT,Grant M
    Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance.
    Plant J., 2006. 46(1): p. 14-33
    [PMID:16553893]
  14. He P, et al.
    Specific bacterial suppressors of MAMP signaling upstream of MAPKKK in Arabidopsis innate immunity.
    Cell, 2006. 125(3): p. 563-75
    [PMID:16678099]
  15. Mosher RA,Durrant WE,Wang D,Song J,Dong X
    A comprehensive structure-function analysis of Arabidopsis SNI1 defines essential regions and transcriptional repressor activity.
    Plant Cell, 2006. 18(7): p. 1750-65
    [PMID:16766691]
  16. Tosti N, et al.
    Gene expression profiles of O3-treated Arabidopsis plants.
    Plant Cell Environ., 2006. 29(9): p. 1686-702
    [PMID:16913859]
  17. Lee DJ, et al.
    Genome-wide expression profiling of ARABIDOPSIS RESPONSE REGULATOR 7(ARR7) overexpression in cytokinin response.
    Mol. Genet. Genomics, 2007. 277(2): p. 115-37
    [PMID:17061125]
  18. Qutob D, et al.
    Phytotoxicity and innate immune responses induced by Nep1-like proteins.
    Plant Cell, 2006. 18(12): p. 3721-44
    [PMID:17194768]
  19. Lee KP,Kim C,Landgraf F,Apel K
    EXECUTER1- and EXECUTER2-dependent transfer of stress-related signals from the plastid to the nucleus of Arabidopsis thaliana.
    Proc. Natl. Acad. Sci. U.S.A., 2007. 104(24): p. 10270-5
    [PMID:17540731]
  20. Libault M,Wan J,Czechowski T,Udvardi M,Stacey G
    Identification of 118 Arabidopsis transcription factor and 30 ubiquitin-ligase genes responding to chitin, a plant-defense elicitor.
    Mol. Plant Microbe Interact., 2007. 20(8): p. 900-11
    [PMID:17722694]
  21. Chawade A,Br
    Putative cold acclimation pathways in Arabidopsis thaliana identified by a combined analysis of mRNA co-expression patterns, promoter motifs and transcription factors.
    BMC Genomics, 2007. 8: p. 304
    [PMID:17764576]
  22. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  23. van Verk MC,Bol JF,Linthorst HJ
    WRKY transcription factors involved in activation of SA biosynthesis genes.
    BMC Plant Biol., 2011. 11: p. 89
    [PMID:21595875]
  24. Gaudinier A, et al.
    Enhanced Y1H assays for Arabidopsis.
    Nat. Methods, 2011. 8(12): p. 1053-5
    [PMID:22037706]
  25. Moreau M, et al.
    EDS1 contributes to nonhost resistance of Arabidopsis thaliana against Erwinia amylovora.
    Mol. Plant Microbe Interact., 2012. 25(3): p. 421-30
    [PMID:22316300]
  26. Hu Y,Dong Q,Yu D
    Arabidopsis WRKY46 coordinates with WRKY70 and WRKY53 in basal resistance against pathogen Pseudomonas syringae.
    Plant Sci., 2012. 185-186: p. 288-97
    [PMID:22325892]
  27. Renault H, et al.
    γ-Aminobutyric acid transaminase deficiency impairs central carbon metabolism and leads to cell wall defects during salt stress in Arabidopsis roots.
    Plant Cell Environ., 2013. 36(5): p. 1009-18
    [PMID:23148892]
  28. Efroni I, et al.
    Regulation of leaf maturation by chromatin-mediated modulation of cytokinin responses.
    Dev. Cell, 2013. 24(4): p. 438-45
    [PMID:23449474]
  29. Ding ZJ,Yan JY,Xu XY,Li GX,Zheng SJ
    WRKY46 functions as a transcriptional repressor of ALMT1, regulating aluminum-induced malate secretion in Arabidopsis.
    Plant J., 2013. 76(5): p. 825-35
    [PMID:24118304]
  30. Ding ZJ, et al.
    Transcription factor WRKY46 regulates osmotic stress responses and stomatal movement independently in Arabidopsis.
    Plant J., 2014. 79(1): p. 13-27
    [PMID:24773321]
  31. Ding ZJ, et al.
    Transcription factor WRKY46 modulates the development of Arabidopsis lateral roots in osmotic/salt stress conditions via regulation of ABA signaling and auxin homeostasis.
    Plant J., 2015. 84(1): p. 56-69
    [PMID:26252246]